r-zz tun-2 Gather alt materials haters proceedlng with the tab. w \\I.’gt; t at AM Procedurgs mw’t SM,- XT’V’A-l a .L (‘Iwa ,3 “V; I3 3W3) ‘…

what is the purpose of this experiment and what are we trying to figure out?r-zz" tun-2 Gather alt materials haters proceedlng with the tab. w \\I.‘> t at AMProcedurgs mw‘t» SM,»- XT‘V‘A-l a .L (‘Iwa ,3 ”V; I3 3W3) ‘1. prepare the spamruphotomeieir” ake sure the spectrop otom r —;-; I Turned on.Set to 600 rim. 0- Set to measure % transmittance [%Ti-. Warmed up. . Then zeroed. h‘ I u3“ Your Instructor may asset you. 2. Prepare the test tubes. . Labei four test tubes: B. 1. 2. and 3‘ fa mfimfifi- Use smelt pieces of tape for labels, and 91363 them 0" the test [aha fig: each tube.7A“? Do not write on the tubes themselves.W Do not place tape on the tubes. ,. . . v- eeveraltirnesto‘rr‘i.. -e the sides of the tube with a Kimwipe. pl’ spectrophotometer (i.e. spec), close the spec lid and pthe instrument. 4. Prepare the treatment test tubes – Measure the buffer, DPIP and mitochondrialsusTable 1 as a guide. W Do not add the suecihate yet!_.-‘ When you are ready, mix Tube 1 win ’close the lid, and measu ‘2/ Record this readingi

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